Increased Levels of 8-Hydroxydeoxyguanosine and Malondialdehyde and its Relationship with Antioxidant Enzymes in Saliva of Periodontitis Patients

نویسندگان

  • Cenk Fatih Canakci
  • Yasin Cicek
  • Abdulkadir Yildirim
  • Ufuk Sezer
  • Varol Canakci
چکیده

OBJECTIVES The aim of this study was to evaluate 8-hydroxydeoxyguanosine (8-OHdG) and Malondialdehyde (MDA) levels, and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in whole saliva of patients with chronic periodontitis. Moreover, the relationship among the oxidative damage biomarkers, antioxidant enzymes activities and clinical periodontal status were investigated. METHODS Whole saliva samples were collected from 30 patients with chronic periodontitis and 30 periodontally healthy control. To determine the clinical condition of each subject, the plaque index, gingival index, clinical attachment level, and probing depth were measured. The salivary 8-OHdG level was measured using the ELISA method. SOD and GPx activities and MDA levels were determined spectrophotometrically. RESULTS Higher salivary 8-OHdG and MDA levels (P<.001), and lower salivary SOD and GPx activities (P<.05) were detected in periodontitis patients compared to the healthy controls. Additionally, there were significant negative correlations between salivary levels of 8-OHdG and both salivary SOD and GPx activities as well as between salivary levels of MDA and both salivary SOD and GPx activities (P<.001). CONCLUSIONS Higher salivary 8-OHdG and MDA levels and lower salivary antioxidant activities seem to reflect increased oxygen radical activity during periodontal inflammation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Determination of Total Antioxidant Capacity in Saliva of Smokers and Non-smokers with Chronic Periodontitis

Background: Periodontal diseases in smokers are 2.6 to 6 times higher than non-smokers and their response to treatment is much lower. Reducing levels of antioxidants and consequently increasing oxidative damage in the pathogenesis of periodontitis is effective. Regarding the importance of free radicals in the pathogenesis of periodontal diseases, the aim of this study was to investigate the eff...

متن کامل

Evaluation of Salivary and Serum Antioxidant and Oxidative Stress Statuses in Patients with Chronic Periodontitis: A Case-Control Study

Aim: Local bacteria stimulate polymorphonuclear neutrophils to release reactive oxygen species in periodontitis. Increased levels of oxidative stress play a significant role in the pathogenesis of periodontitis. Therefore, this study aimed to evaluate total salivary and serum antioxidant capacity and malondialdehyde in patients with chronic periodontitis. Materials and methods: Fifty-five healt...

متن کامل

Comparison of cytokines IL10, IFN-Y, TNF-α, TGF-β and antioxidant enzymes glutathione reductase and glutathione peroxidase in saliva and serum of Iranian patients with alopecia totalis

The aim of this study was to investigate the possibility of using saliva instead of serum (as a simpler method without the need for blood sampling) to determine the level of some cytokines and enzymes. In this study, the levels of cytokines IL10, IFN-Y, TNF-α, TGF-β and the antioxidant enzymes glutathione reductase and glutathione peroxidase were measured in the saliva and serum of three patien...

متن کامل

Antioxidant Enzymes and Acute Phase Proteins Correlate with Marker of Lipid Peroxide in Adult Nigerian Sickle Cell Disease Patients

Objective(s) Sickle cell disease is a genetic disorder characterized by chronic haemolytic anaemia. Haemoglobin S containing red blood cells may be susceptible to oxidative stress due to imbalance between production of reactive oxygen species and the countering effect of the various antioxidants present in the body. Materials and Methods We evaluated some antioxidant enzymes which include gl...

متن کامل

Increased Salivary Levels of 8-Hydroxydeoxyguanosine May Be a Marker for Disease Activity for Periodontitis

BACKGROUND 8-hydroxydeoxyguanosine (8-OHdG) is commonly used as a marker to evaluate oxidative DNA damage in disorders including chronic inflammatory diseases such as inflammatory periodontal pathologies. In the current study we hypothesized that the level of 8-OHdG in saliva increases by the periodontal destruction severity determined by clinical parameters as clinical attachment level (CAL). ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • European Journal of Dentistry

دوره 3  شماره 

صفحات  -

تاریخ انتشار 2009